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A role for nitric oxide-mediated glandular hypofunction in a non-apoptotic model for Sjögren’s syndrome

Caulfield, Vicky L.; Balmer, Colette; Dawson, Luke J. and Smith, Peter M. (2009) A role for nitric oxide-mediated glandular hypofunction in a non-apoptotic model for Sjögren’s syndrome. Rheumatology, 48 (7). pp. 727-733. ISSN 1462-0324

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Abstract

Objective To investigate a role for the inflammatory mediator, nitric oxide (NO) in Sjögren’s syndrome, an autoimmune condition characterised by salivary and lacrimal gland hypofunction resulting from failure of acinar cells to secrete. Methods FURA-2 microfluorimetry was used to measure agonist evoked changes of [Ca2+]i in isolated mouse and human salivary acinar cells following exposure to NO donors. Results NO had a biphasic effect on salivary acinar function. Acute exposure to NO (2 minutes) caused a cyclic GMP-dependent, ODQ-sensitive increase in the Ca2+ signal elicited in response to ACh stimulation, consistent with stimulation of Ryanodine receptors by cyclic ADP ribose. Prolonged exposure to NO (>40 minutes) significantly reduced the ACh-evoked Ca2+ signal by a mechanism independent of cyclic GMP. We found no differences between the responses of human and mouse acinar cells. Conclusion Our data show that chronic exposure to NO, which is known to be elevated in Sjögren’s syndrome, could have a role in salivary gland hypofunction. We note a similarity in the response to stimulation of salivary acinar exposed to NO and that which we have previously reported in salivary acinar cells isolated from patients with Sjögren’s syndrome. We speculate that NO mediated nitrosylation of one or more elements of the signal transduction pathway could underlie down-regulation of salivary function in Sjögren’s syndrome.

Item Type:Article
Additional Information:Rheumatology Advance Access originally published online on May 8, 2009
Uncontrolled Keywords:Sjögren's syndrome; autoimmune; Nitric Oxide; salivary; secretion; Ca2+
Subjects:R Medicine > RK Dentistry
Departments, Research Centres and Related Units:Academic Faculties, Institutes and Research Centres > Faculty of Medicine > School of Dental Sciences
DOI:10.1093/rheumatology/kep100
Publisher's Statement:This is a pre-copy-editing, author-produced PDF of an article accepted for publication in Rheumatology following peer review. The definitive publisher-authenticated version (Vicky L. Caulfield , Colette Balmer , Luke J. Dawson , and Peter M. Smith A role for nitric oxide-mediated glandular hypofunction in a non-apoptotic model for Sjögren's syndrome Rheumatology Advance Access published on July 1, 2009, DOI 10.1093/rheumatology/kep100. Rheumatology 48: 727-733) is available online at: http://dx.doi.org/10.1093/rheumatology/kep100
Refereed:Yes
Status:Published
ID Code:1166
Deposited On:16 Oct 2009 10:18
Last Modified:19 May 2011 18:11

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