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The inability of a bacteriophage to infect Staphylococcus aureus does not prevent it from specifically delivering a photosensitiser to the bacterium enabling its lethal photosensitisation

Hope, C.K.; Packer, S.; Wilson, M. and Nair, S.P. (2009) The inability of a bacteriophage to infect Staphylococcus aureus does not prevent it from specifically delivering a photosensitiser to the bacterium enabling its lethal photosensitisation. Journal of Antimicrobial Chemotherapy, 64 (1). pp. 59-61. ISSN 1460-2091 (Online); 0305-7453 (Print)

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Abstract

Objectives: It has been demonstrated that the efficiency of lethal photosensitisation can be improved by covalently binding photosensitising agents to bacteriophage. In this study we have investigated whether a bacteriophage requires the capacity to infect the bacterium to enhance lethal photosensitisation when linked to a photosensitizer. Methods: Tin (IV) chlorin e6 (SnCe6) was conjugated to bacteriophage Φ11, a transducing phage which can infect Staphylococcus aureus NCTC 8235-4, but not EMRSA 16. The conjugate and appropriate controls, were incubated with these bacteria and either exposed to laser light at 632.8 nm or kept in the dark. Results: The SnCe6 / Φ11 conjugate achieved a statistically significant reduction in the number of viable bacteria of both 8325-4 and EMRSA 16 strains by 2.31 log10 and 2.63 log10 respectively. The conjugate could not however instigate lethal photosensitisation in E. coli. None of the other combinations of controls; such as an equivalent concentration of SnCe6 only, an equivalent titre of bacteriophage only or experiments conducted without laser light; yielded significant reductions in the number of viable bacteria recovered. Conclusions: The inability of a bacteriophage to infect S. aureus does not prevent it from specifically delivering a photosensitiser to a bacterium enabling its lethal photosensitisation.

Item Type:Article
Additional Information:Advance Access publication 2 May 2009
Uncontrolled Keywords:Tin (IV) chlorin e6 (SnCe6), MRSA, Photodynamic therapy, PDT
Subjects:R Medicine > RK Dentistry
Departments, Research Centres and Related Units:Academic Faculties, Institutes and Research Centres > Faculty of Medicine > School of Dental Sciences
DOI:10.1093/jac/dkp157
Publisher's Statement:This is a pre-copy-editing, author-produced PDF of an article accepted for publication in Journal of Antimicrobial Chemotherapy following peer review. The definitive publisher-authenticated version 'C. K. Hope , S. Packer , M. Wilson , and S. P. Nair The inability of a bacteriophage to infect Staphylococcus aureus does not prevent it from specifically delivering a photosensitizer to the bacterium enabling its lethal photosensitization JAC Advance Access published on July 1, 2009, DOI 10.1093/jac/dkp157. J. Antimicrob. Chemother. 64: 59-61.' is available online at: http://dx.doi.org/doi:10.1093/jac/dkp157
Refereed:Yes
Status:Published
ID Code:1169
Deposited On:16 Oct 2009 10:25
Last Modified:18 Feb 2013 16:11

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