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The evaluation of a novel method comparing quantitative light-induced fluorescence (QLF) with spectrophotometry to assess staining and bleaching of teeth

Adeyemi, A.A.; Jarad, F.D.; de Josselin de Jong, E.; Pender, N. and Higham, S.M. (2010) The evaluation of a novel method comparing quantitative light-induced fluorescence (QLF) with spectrophotometry to assess staining and bleaching of teeth. Clinical Oral Investigations, 14 (1). pp. 19-25. ISSN 1436-3771 (Online); 1432-6981 (Print)

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Abstract

Title: The evaluation of a novel method comparing Quantitative Light-induced Fluorescence (QLF) with spectrophotometry to assess staining and bleaching of teeth. Objectives: This study reports the development and evaluation of a novel method using QLF which enables its use for quantifying and assessing whole tooth surface staining and tooth whitening. The method was compared with a spectrophotometer to assess reliability. Methods: Two experimental phases, intrinsic stain formation and tooth whitening were conducted in vitro on sixteen extracted bovine teeth. Intrinsic stains were developed via access through lingual surfaces and root canals of these teeth using tea solution (2 g/100 ml, Marks and Spencer Extra Strong Tea, Marks and Spencer, London, U.K) for six days. Stains were removed using 33 % hydrogen peroxide (VWR Prolab, Leicestershire, U.K) in cycles over 150 min. Stain development / whitening was monitored with QLF (Inspektor Research systems, Amsterdam, Netherlands) and Spectrophotometry (Easy shade, Vita Zahnfabrik, Bad Säckingen, Germany). Parameters Delta F for QLF and Delta E for the Spectrophotometer were obtained. The progression of stain intensity and removal observed by the methods were tested for correlation using Pearson’s correlation coefficient. Intra examiner reliability for each method was tested. Results: QLF showed a high correlation with spectrophotometry for detecting and monitoring intrinsic tooth stain progression (Pearson coefficient r was -0.987 with correlation significant p < 0.0001. For stain removal the Pearson coefficient (r) between both methods was -0.906 with no significance p = 0.094. Conclusion: The use of an external reference material in combination with the inner patch QLF analysis technique had the ability to detect and measure whole tooth surface staining and its removal longitudinally. The reliability of the method shows a potential clinical application.

Item Type:Article
Additional Information:Available online: 21 March 2009. Issue: February 2010.
Uncontrolled Keywords:QLF; Fluorescence; Spectrophotometry; Staining; Tooth Whitening
Subjects:R Medicine > RK Dentistry
Departments, Research Centres and Related Units:Academic Faculties, Institutes and Research Centres > Faculty of Medicine > School of Dental Sciences
DOI:10.1007/s00784-009-0261-9
Publisher's Statement:© Springer-Verlag 2009. This article was first published in Adeyemi, A. A.; Jarad, F. D.; de Josselin de Jong, E.; Pender, N. and Higham, S. M. (2010) The evaluation of a novel method comparing quantitative light-induced fluorescence (QLF) with spectrophotometry to assess staining and bleaching of teeth. Clinical Oral Investigations, 14(1), pp. 19-25. The final publication is available at www.springerlink.com .
Refereed:Yes
Status:Published
ID Code:1176
Deposited On:08 Jun 2009 10:03
Last Modified:22 Feb 2012 12:38

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