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A direct comparison between extracted tooth and filter-membrane biofilm models of endodontic irrigation using Enterococcus faecalis

Hope, Christopher K.; Garton, Stuart G.; Wang, Qian; Burnside, Girvan and Farrelly, Peter J. (2010) A direct comparison between extracted tooth and filter-membrane biofilm models of endodontic irrigation using Enterococcus faecalis. Archives of Microbiology, 192 (9). pp. 775-781. ISSN 1432-072X (Online); 0302-8933 (Print)

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Abstract

Endodontic restorations often fail due to inadequate disinfection of the root canal even though the antimicrobial irrigants used have been shown to be capable of killing the bacterium frequently implicated in this complication, Enterococcus faecalis (Ef). Extracted human teeth were root-prepared and filled with a liquid culture of Ef. Following incubation, the root canals were irrigated with 1% sodium hypochlorite (NaOCl), electrochemically activated water or saline control. Irrigation was modelled using an electronic pipette to deliver the solutions at a reproducible flow velocity. A series of parallel experiments employed a membrane biofilm model that was directly immersed into irrigant. Experimental conditions where contiguous between the extracted tooth model and biofilm model wherever possible. 1% NaOCl effectively sterilised the biofilm model after 60 seconds exposure, whereas log 3.36 viable Ef where recoverable from the analogous extracted tooth model. Biofilms of Ef were susceptible to concentrations of irrigant that proved ineffective in the tooth model. NaOCl was the most effective biocide in either case. This suggests that the biofilm modality of bacterial growth is not important for the recalcitrance of root canal infections following endodontic irrigation; it is more likely due to the inability of the irrigant to access the infection.

Item Type:Article
Additional Information:Available online 23 July 2010. Issue date: September 2010. Title on uploaded document is different to the published PDF. Title: A direct comparison between extracted tooth and biofilm models of endodontic irrigation using Enterococcus faecalis
Uncontrolled Keywords:Enterococcus faecalis; In vitro modelling; Hypochlorous acid; Sodium hypochlorite
Subjects:R Medicine > RK Dentistry
Departments, Research Centres and Related Units:Academic Faculties, Institutes and Research Centres > Faculty of Medicine > School of Dental Sciences
DOI:10.1007/s00203-010-0604-6
Publisher's Statement:© Springer-Verlag 2010. This article was first published in Hope, Christopher K.; Garton, Stuart G.; Wang, Qian; Burnside, Girvan. and Farrelly, Peter J. (2010) A direct comparison between extracted tooth and filter-membrane biofilm models of endodontic irrigation using Enterococcus faecalis. Archives of Microbiology, 192 (9). pp. 775-781. http://dx.doi.org/10.1007/s00203-010-0604-6. The final publication is available at www.springerlink.com .
Refereed:Yes
Status:Published
ID Code:1386
Deposited On:01 Sep 2010 10:39
Last Modified:16 Dec 2011 16:02

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