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Scholarly Communication

Effects of zinc and fluoride on the remineralisation of artificial carious lesions under simulated plaque-fluid conditions

Lynch, R.J.M.; Churchley, D.; Butler, A.; Kearns, S.; Thomas, G.V.; Badrock, T.C.; Cooper, L. and Higham, S.M. (2011) Effects of zinc and fluoride on the remineralisation of artificial carious lesions under simulated plaque-fluid conditions. Caries Research, 45 (3). pp. 313-322. ISSN 1421-976X (Online); 0008-6568 (Print)

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Cited 5 times in WoS


The aim was to study the effects of zinc and fluoride on remineralisation at plaque-fluid (PF) concentrations. Artificial carious lesions were created in two acid-gel demineralising systems (initially infinitely under-saturated and partially-saturated with respect to enamel) giving lesions with different mineral distribution characteristics (high- and low-R respectively) but similar integrated mineral-loss values. Lesions of both types were assigned to one of four groups and remineralised for 5 d at 37°C. Zinc and fluoride were added, based on PF concentrations 1 h post-application, to give four treatments; 231 µmol/L zinc (Zn), 10.5 µmol/L fluoride (F), zinc/fluoride combined (Zn/F) and an unmodified control solution (non-F/non-Zn). Subsequently remineralisation was measured using microradiography. High-R lesions were analysed for calcium, phosphorus, fluoride and zinc using electron-probe microanalysis (EPMA). All lesions underwent statistically-significant remineralisation. For low-R, remineralisation was in the order non-F/non-Zna < Fa < Znab < Zn/Fb, and for high-R, Fa < non-F/non-Znb < Znb < Zn/Fc (treatments with the same letter not significantly different (p < 0.05)). Qualitatively, remineralisation occurred throughout non-F/non-Zn and Zn, predominantly at the surface-zone (F) and within the lesion-body (Zn/F). EPMA revealed zinc in relatively large amounts in the outer regions (Zn, Zn/F). Fluoride was abundant not only at the surface (F) but also in the lesion-body (Zn/F). Calcium:phosphate ratios were similar to hydroxyapatite (all). To conclude, under static remineralising conditions simulating PF, Zn/F gave significantly greater remineralisation than did F, possibly because zinc in Zn/F maintained greater surface-zone porosity when compared with F, facilitating greater lesion-body remineralisation.

Item Type:Article
Additional Information:Published online June 30, 2011. Issue date : July 2011. Cited as : Lynch RJM, Churchley D, Butler A, Kearns S, Thomas GV, Badrock TC, Cooper L, Higham SM: Effects of Zinc and Fluoride on the Remineralisation of Artificial Carious Lesions under Simulated Plaque Fluid Conditions. Caries Res 2011;45:313-322 (DOI: 10.1159/000324804)
Uncontrolled Keywords:Remineralisation; lesion; zinc; fluoride
Subjects:R Medicine > RK Dentistry
Departments, Research Centres and Related Units:Academic Faculties, Institutes and Research Centres > Faculty of Medicine > School of Dental Sciences
Publisher's Statement:© 2011 S. Karger AG, Basel
ID Code:1481
Deposited On:04 Aug 2011 10:46
Last Modified:01 Jun 2012 14:49

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