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HmuY haemophore and gingipain proteases constitute a unique syntrophic system of haem acquisition by Porphyromonas gingivalis

Smalley, John W.; Byrne, Dominic P.; Birss, Andrew J.; Wojtowicz, Halina; Sroka, Aneta; Potempa, Jan and Olczak, Teresa (2011) HmuY haemophore and gingipain proteases constitute a unique syntrophic system of haem acquisition by Porphyromonas gingivalis. PLoS ONE, 6 (2). Article Number: e17182. ISSN 1932-6203

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Abstract

Haem (iron protoporphyrin IX) is both an essential growth factor and virulence regulator for the periodontal pathogen Porphyromonas gingivalis, which acquires it mainly from haemoglobin via the sequential actions of the R- and K-specific gingipain proteases. The haem-binding lipoprotein haemophore HmuY and its cognate receptor HmuR of P. gingivalis, are responsible for capture and internalisation of haem. This study examined the role of the HmuY in acquisition of haem from haemoglobin and the cooperation between HmuY and gingipain proteases in this process. Using UV-visible spectroscopy and polyacrylamide gel electrophoresis, HmuY was demonstrated to wrest haem from immobilised methaemoglobin and deoxyhaemoglobin. Haem extraction from oxyhaemoglobin was facilitated after oxidation to methaemoglobin by pretreatment with the P. gingivalis R-gingipain A (HRgpA). HmuY was also capable of scavenging haem from oxyhaemoglobin pre-treated with the K-gingipain (Kgp). This is the first demonstration of a haemophore working in conjunction with proteases to acquire haem from haemoglobin. In addition, HmuY was able to extract haem from methaemalbumin, and could bind haem, either free in solution or from methaemoglobin, even in the presence of serum albumin.

Item Type:Article
Additional Information:Formal correction (PLoS ONE). Article has been formally corrected to address the following errors. 1) The first affiliation is incorrect. It should read "Department of Clinical Infection, Microbiology and Immunology, Institute of Infection and Global Health, University of Liverpool, Liverpool, United Kingdom." 2) There is an error in the third sentence of the funding section. The sentence should read: "J.W.S. is grateful to the School of Dentistry, University of Liverpool, for financial support for D.P.B." Published online February 17, 2011. 10 pages. Citation: Smalley JW, Byrne DP, Birss AJ, Wojtowicz H, Sroka A, et al. (2011) HmuY Haemophore and Gingipain Proteases Constitute a Unique Syntrophic System of Haem Acquisition by Porphyromonas gingivalis. PLoS ONE 6(2): e17182. doi:10.1371/journal.pone.0017182.
Uncontrolled Keywords:PORPHYROMONAS GINGIVALIS; GINGIPAIN; ROTOPORPHYRIN-IX; HAEMOGLOBIN; PROTEINS
Subjects:R Medicine > RK Dentistry
Departments, Research Centres and Related Units:Academic Faculties, Institutes and Research Centres > Faculty of Medicine > School of Dental Sciences
DOI:10.1371/journal.pone.0017182
Publisher's Statement:© 2011 Smalley et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Related URLs:
Refereed:Yes
Status:Published
ID Code:1500
Deposited On:16 May 2011 16:03
Last Modified:19 May 2011 10:52

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