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Cytokine mRNA profiling of peripheral blood mononuclear cells (PBMC) from trypanotolerant and trypanosusceptible cattle infected with Trypanosoma congolense

O'Gorman, Grace M.; Park, Stephen D. E; Hill, Emmeline W.; Meade, Kieran G.; Mitchell, Laura C.; Agaba, Morris; Gibson, John P.; Hanotte, Olivier; Naessens, Jan; Kemp, Stephen J. and McHugh, David E. (2006) Cytokine mRNA profiling of peripheral blood mononuclear cells (PBMC) from trypanotolerant and trypanosusceptible cattle infected with Trypanosoma congolense. Physiological Genomics, 28 . pp. 53-61. ISSN 1094-8341

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Abstract

To examine differences in cytokine profiles that may confer tolerance/susceptibility to bovine African trypanosomiasis, N’Dama (trypanotolerant, n = 8) and Boran (trypanosusceptible, n = 8) cattle were experimentally challenged with Trypanosoma congolense. Blood samples were collected over a 34 day period and RNA was extracted from peripheral blood mononuclear cells (PBMC). The expression levels of a panel of 14 cytokines were profiled over the time course of infection and between breeds. Messenger RNA (mRNA) transcript levels for the IL2, IL8 and IL1RN genes were significantly downregulated across the time course of infection in both breeds. There was an early increase in transcripts for genes encoding proinflammatory mediators (IFNG, IL1A, TNF and IL12) in N’Dama by 14 days post infection (dpi) compared to pre-infection levels that was not detected in the susceptible Boran breed. By the time of peak parasitemia, a TH2-like cytokine environment was prevalent, which was particularly evident in the Boran. Increases in transcripts for the IL6 (29 and 34 dpi) and IL10 (21, 25 and 29 dpi) genes were detected that were higher in the Boran compared to N’Dama. These findings highlight the implications for using murine models to study the bovine immune response to trypanosomiasis, where in some cases cytokine expression patterns differ. Overall, these data suggest that the trypanotolerant N’Dama are more capable of responding very early in infection with proinflammatory and TH1 type cytokines than the trypanosusceptible Boran and may explain why N’Dama control parasitemia more efficiently than Boran during the early stages of infection.

Item Type:Article
Additional Information:First published September 19, 2006.
Uncontrolled Keywords:trypanosomiasis; gene expression; quantitative real-time RT-PCR; BOVINE LYMPHOCYTES-T; AFRICAN TRYPANOSOMIASIS; MONOCLONAL-ANTIBODIES; BORAN CATTLE; ANTIGENIC VARIATION; INTERFERON-GAMMA; NITRIC-OXIDE; TNF-ALPHA; RESISTANCE; NDAMA
Subjects:Q Science > Q Science (General)
Q Science > QP Physiology
Departments, Research Centres and Related Units:Academic Faculties, Institutes and Research Centres > Faculty of Science > Department of Biological Sciences
DOI:10.1152/physiolgenomics.00100.2006
Refereed:Yes
Status:Published
ID Code:246
Deposited On:12 Aug 2008 10:32
Last Modified:20 May 2011 18:36

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