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Congenic mice infected with Trypanosoma congolense

Noyes, Harry; Agaba, Morris; Anderson, Susan; Brass, Andy; Broadhead, Anthea; Fisher, Paul; Hulme, Helen; Kemp, Stephen; Rennie, Catriona; Withers, Jennifer; Zeef, Leo; Wardlesworth, Leanne; Gibson, John; Hanotte, Olivier; Musa, Hassan; Mwakaya, Joel; Mwangi, Daniel; Nganga, Joseph; Ogugo, Moses; Wambugu, John; Daly, Daly and Thomas, Luke (2007) Congenic mice infected with Trypanosoma congolense. In: Mouse Genetics Conference, 05-09 September 2007, Wellcome Trust Conference Centre, Hinxton Hall, Hinxton, UK. (Unpublished)

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Abstract

There is still no well-defined strategy for discovering the Quantitive Trait Genes (QTG) amongst the hundreds of candidates within underlying Quantitive Trait Loci (QTL). We have created three congenic mouse lines that have C57BL/6 regions covering QTL for response to infection with Trypanosoma congolense introgressed into an A/J background. We have undertaken a systematic analysis of the effect of SNP, expression polymorphisms and genomic rearrangements within the congenic regions on genome wide gene and protein expression. This data has been used to identify the genes and pathways regulated by the congenic region and at the same time identify genetic polymorphisms within the region that could plausibly regulate the differences in genome wide gene expression that we have observed. All classes of polymorphism within the QTL had detectable effects on both cis and trans gene expression. A structural polymorphism within Notch4 within the QTL was correlated with altered expression of Dll4 a ligand of Notch4 on a different chromosome. A genomic duplication within the QTL was associated with altered expression of GloI within the duplicated region. Analysis of pathways that had altered gene expression and that also contained genes within the QTL identified showed that changes in expression of genes involved in apoptosis were correlated with an amino acid indel in Daxx. Analysis of transcription factor binding sites within differentially expressed genes identified transcription factors that might mediate the trans regulation of gene expression.

Item Type:Conference or Workshop Item (Other)
Uncontrolled Keywords:Congolense; GENE; gene expression; genetics; Mice; Mouse; poster; Presentation; Trypanosoma; TRYPANOSOMA-CONGOLENSE; Trypanosoma congolense
Subjects:Q Science > QH Natural history > QH301 Biology
Q Science > QH Natural history > QH426 Genetics
Departments, Research Centres and Related Units:Academic Faculties, Institutes and Research Centres > Faculty of Science > Department of Biological Sciences
Refereed:No
Status:Unpublished
ID Code:322
Deposited On:20 Apr 2010 14:36
Last Modified:20 May 2011 17:17

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