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Alterations of endometrial basement membrane integrity and stem/progenitor cell markers in endometriosis

Palial, Kanchan (2011) Alterations of endometrial basement membrane integrity and stem/progenitor cell markers in endometriosis. Masters thesis, University of Liverpool.

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Abstract

Alterations of Endometrial Basement Membrane Integrity and Stem/Progenitor Cell Markers in Endometriosis Palial, K; Drury, J, Heathcote, L, Valentijin, A.J, Gazvani, R, Rudland, P.S. and Hapangama, D.K. Department of Women's and Children's Health, University of Liverpool, Liverpool, United Kingdom, L8 7SS. School of Biological Sciences, University of Liverpool, Liverpool, United Kingdom, L69 7ZB Degradation of basement membrane (BM) is reported to be involved in the metastatic process of cancers, and has been shown to be aberrantly expressed in the benign metastatic disease of endometriosis. Emerging evidence show endometrial stem/progenitor cells are involved in endometrial physiology and in certain endometrial pathological conditions, however markers for these cells are lacking. We tested a panel of stem cell markers including cytokeratin 5/6 (CK5/6) and podocalyxin-like protein (PODXL) and stage specific embryonic antigen 1 (SSEA1) in endometrial tissue. Using immunohistochemistry, we aim to investigate if an alteration of BM integrity is associated with differential expression of these stem cell markers in eutopic endometrium collected from fertile controls, post-menopausal (PM) women and patients with endometriosis and endometrial cancer. 1. In the fertile control group, expression of BM components, Collagen IV and laminin, changed across the menstrual cycle and some of these changes agree with previously reported findings. Expression of stem cell markers SSEA1 and PODXL also showed similar changes across the menstrual cycle with both having maximal expression during the ProlP. Expression of BM components appeared to be correlated with stem cell markers. No cyclical changes in CK5/6 were reported in fertile controls. 2. In the PM group a few differences were seen in BL integrity compared with the fertile control group, especially in BM supporting endometrial vessels. Nonetheless, PODXL and SSEA1 expression was similar to the fertile control group. 3. Complete disruption of BM in the endometrial cancer group provided confirmatory results that BM is disruption in this invasive metastatic disease. 4. In the endometriosis group, differences were seen in the expression of the key BM components in eutopic endometrium sampled from women with endometriosis when compared with fertile controls, and this was similar to previous reported findings. Whereas SSEA1 showed a phase dependent expression in the fertile control group, this was lost in the endometriosis group.

Item Type:Thesis (Masters)
Subjects:R Medicine > R Medicine (General)
R Medicine > RL Dermatology
Departments, Research Centres and Related Units:Academic Faculties, Institutes and Research Centres > Faculty of Medicine > School of Reproductive & Developmental Medicine
Status:Unpublished
ID Code:5313
Deposited On:07 Aug 2012 11:40
Last Modified:01 Aug 2013 01:00

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