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Induction of lethal photosensitization in biofilms using a confocal scanning laser as the excitation source

Hope, C.K. and Wilson, M. (2006) Induction of lethal photosensitization in biofilms using a confocal scanning laser as the excitation source. Journal of Antimicrobial Chemotherapy, 57 (6). pp. 1227-1230. ISSN 0305-7453

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Official URL: http://jac.oxfordjournals.org

Cited 9 times in WoS

Abstract

Objectives. To induce lethal photosensitisation in biofilms of Streptococcus pyogenes by using the scanning laser in a confocal microscope to photoactivate Sn (IV) chlorin e6 (SnCe6) whilst simultaneously measuring changes in cell vitality using fluorescent indicators of membrane integrity. Methods. Biofilms of S. pyogenes were immersed in a solution of 50 mg/L (70.28μM) SnCe6 and scanned with the 488 nm argon and 543 nm HeNe lasers in a confocal microscope. Changes in membrane permeability were quantified using image analysis tools. Results. Cell permeability increased in biofilms of S. pyogenes after successive scanning / exposure cycles in the presence of SnCe6. Conclusions. Cell death was induced in biofilms of S. pyogenes by the photosensitiser SnCe6 on exposure to the scanning laser emissions of a confocal microscope. The simultaneous recording of cell death demonstrates the real-time evaluation of a light-activated antimicrobial compound against biofilm.

Item Type:Article
Additional Information:JAC Advance Access - originally published online on March 20, 2006. Issue: June 2006.
Uncontrolled Keywords:BIOFILM; PHOTODYNAMIC; THERAPY; STREPTOCOCCUS; PYOGENES; SN (IV) CHLORINE6; RESISTANT STAPHYLOCOCCUS-AUREUS; PHOTODYNAMIC THERAPY; TOLUIDINE-BLUE; ORAL BACTERIA; LIGHT
Subjects:R Medicine > RK Dentistry
Departments, Research Centres and Related Units:Academic Faculties, Institutes and Research Centres > Faculty of Medicine > School of Dental Sciences
DOI:10.1093/jac/dkl096
Publisher's Statement:This is a pre-copy-editing, author-produced PDF of an article accepted for publication in Journal of Antimicrobial Chemotherapy following peer review. The definitive publisher-authenticated version of Journal of Antimicrobial Chemotherapy 2006 57(6):1227-1230; doi:10.1093/jac/dkl096 is available online at: http://dx.doi.org/doi:10.1093/jac/dkl096
Refereed:Yes
Status:Published
ID Code:68
Deposited On:26 Jun 2007 12:16
Last Modified:22 Feb 2012 11:16

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