Fothergill, Joanne L.; Panagea, Stavroula; Hart, Charles A.; Walshaw, Martin J.; Pitt, Tyrone L. and Winstanley, Craig (2007) Widespread pyocyanin over-production among isolates of a cystic fibrosis epidemic strain. BMC Microbiology, 7 . Article Number: 45. ISSN 1471-2180
Available under License Creative Commons Attribution.
Cited 30 times in WoS
Background: Some isolates of the Liverpool cystic fibrosis epidemic strain of Pseudomonas aeruginosa exhibit an unusual virulence-related phenotype, characterized by over-production of quorum sensing-regulated exoproducts such as pyocyanin and LasA protease. Our aim was to determine the prevalence of this unusual phenotype amongst isolates of the epidemic strain, and to study other intraclonal phenotypic and genotypic variations. Results: The unusual phenotype was detected in at least one epidemic strain isolate from the majority of cystic fibrosis patients tested, and can be retained for up to seven years during chronic infection. Multiple sequential isolates of the epidemic strain taken from six patients over a period of up to nine years exhibited a wide range of phenotypes, including different antimicrobial susceptibilities. Our data suggest that each sputum sample contains a mixture of phenotypes and genotypes within the epidemic strain population, including within colony morphotypes. Many isolates exhibit premature (during early rather than late exponential growth) and over-production of pyocyanin, which has a number of toxic effects directly relevant to cystic fibrosis. Conclusion: The widespread occurrence of this unusual phenotype suggests that it may play an important role in the success of the epidemic strain.
|Additional Information:||10 pages (page numbers not for citation purposes). Published: 23 May 2007.|
|Uncontrolled Keywords:||PSEUDOMONAS-AERUGINOSA PYOCYANIN; AIRWAY EPITHELIAL-CELLS; PHENAZINE-1-CARBOXYLIC ACID; SUBTRACTIVE HYBRIDIZATION; ANTIMICROBIAL RESISTANCE; NEUTROPHIL APOPTOSIS; EXPRESSION; LUNG; INFECTION; MECHANISMS|
|Subjects:||Q Science > QR Microbiology|
|Departments, Research Centres and Related Units:||Academic Faculties, Institutes and Research Centres > Faculty of Medicine > School of Infection & Host Defence|
|Publisher's Statement:||© 2007 Fothergill et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.|
|Deposited On:||25 Jun 2008 14:34|
|Last Modified:||24 Apr 2012 10:28|
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