Hepworth, Philip J.; Leatherbarrow, Howard; Hart, C. Anthony and Winstanley, Craig (2007) Use of suppression subtractive hybridisation to extend our knowledge of genome diversity in Campylobacter jejuni. BMC Genomics, 8 . Article Number: 110. ISSN 1471-2164
Available under License Creative Commons Attribution.
Cited 3 times in WoS
Background: Previous studies have sought to identify a link between the distribution of variable genes amongst isolates of Campylobacter jejuni and particular host preferences. The genomic sequence data available currently was obtained using only isolates from human or chicken hosts. In order to identify variable genes present in isolates from alternative host species, five subtractions between C. jejuni isolates from different sources (rabbit, cattle, wild bird) were carried out, designed to assess genomic variability within and between common multilocus sequence type (MLST) clonal complexes (ST-21, ST-42, ST-45 and ST-61). Results: The vast majority (97%) of the 195 subtracted sequences identified had a best BLASTX match with a Campylobacter protein. However, there was considerable variation within and between the four clonal complexes included in the subtractions. The distributions of eight variable sequences, including four with putative roles in the use of alternative terminal electron acceptors, amongst a panel of C. jejuni isolates representing diverse sources and STs, were determined. Conclusion: There was a clear correlation between clonal complex and the distribution of the metabolic genes. In contrast, there was no evidence to support the hypothesis that the distribution of such genes may be related to host preference. The other variable genes studied were also generally distributed according to MLST type. Thus, we found little evidence for widespread horizontal gene transfer between clonal complexes involving these genes.
|Additional Information:||11 pages (page numbers not for citation purposes). Published: 30 April 2007.|
|Uncontrolled Keywords:||COMPARATIVE PHYLOGENOMICS; SEQUENCE; COLONIZATION; IDENTIFICATION; MICROARRAY; VIRULENCE; GENES; RESPIRATION; PHENOTYPES; NITRITE|
|Subjects:||R Medicine > R Medicine (General)|
|Departments, Research Centres and Related Units:||Academic Faculties, Institutes and Research Centres > Faculty of Medicine > School of Infection & Host Defence|
Academic Faculties, Institutes and Research Centres > Faculty of Veterinary Science > Department of Veterinary Clinical Science
|Publisher's Statement:||© 2007 Hepworth et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.|
|Deposited On:||25 Jun 2008 15:23|
|Last Modified:||04 May 2012 12:42|
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