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Biofilm structure and cell vitality in a laboratory model of subgingival plaque

Hope, C.K. and Wilson, M. (2006) Biofilm structure and cell vitality in a laboratory model of subgingival plaque. Journal of Microbiological Methods, 66 (3). pp. 390-398. ISSN 0167-7012

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Cited 6 times in WoS

Abstract

The accumulation of dental plaque below the gingival margin (i.e. subgingival plaque) is responsible for the most prevalent microbe-induced diseases of humans — the periodontal diseases. Access to this plaque is difficult, making studies of its structure in vivo very difficult. We have, therefore, used a constant-depth film fermenter to grow microcosm subgingival dental plaques under conditions similar to those existing in vivo to enable us to study certain aspects of its structure. Confocal laser scanning microscopy revealed that the biofilms consisted of pillar-like stacks of bacteria separated by water channels. In terms of their structure, these microcosm dental plaques reached a pseudo steady-state after 4 days. Individual optical sections generally showed the outer layers of the biofilm stacks to contain a high proportion of nonviable cells surrounding an inner core of predominantly viable cells with ‘veins’ of nonviable bacteria penetrating from the outer layers through into the inner core. Such a structure differs from that classically described for biofilms growing in an aerobic atmosphere where the arrangement of viable and nonviable bacteria is usually reversed. The reasons for the preponderance of nonviable bacteria in the outer layer of the bacterial stacks remain to be established.

Item Type:Article
Additional Information:Available online 17 February 2006. Issue: September 2006.
Uncontrolled Keywords:SUBGINGIVAL PLAQUE; ANAEROBIC BIOFILM; CONFOCAL LASER SCANNING MICROSCOPY; VIABILITY; CONSTANT-DEPTH FILM FERMENTER; HUMAN PERIODONTAL POCKETS; LASER-SCANNING MICROSCOPY; MICROCOSM DENTAL PLAQUES; BACTEROIDES-FORSYTHUS; SUPRAGINGIVAL PLAQUE; SPATIAL-DISTRIBUTION; BACTERIAL BIOFILMS; CREVICULAR FLUID; OXYGEN-TENSION; FILM FERMENTER
Subjects:R Medicine > RK Dentistry
Departments, Research Centres and Related Units:Academic Faculties, Institutes and Research Centres > Faculty of Medicine > School of Dental Sciences
DOI:10.1016/j.mimet.2006.01.003
Refereed:Yes
Status:Published
ID Code:72
Deposited On:09 Aug 2007 17:14
Last Modified:22 Feb 2012 11:16

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