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Saliva collection methods for DNA biomarkers in oral cancer patients

Matthews, April M; Kaur, Harpal; Dodd, Michael; D'Souza, Jacob; Liloglou, Triantafillos; Shaw, Richard J and Risk, Janet M (2013) Saliva collection methods for DNA biomarkers in oral cancer patients. British Journal of Oral and Maxillofacial Surgery, 15 (5). pp. 394-398. ISSN 1532-1940 (Online); 0266-4356 (Print)

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Abstract

Patients with head and neck cancers are predisposed to local recurrence and second primaries due to the phenomenon of field cancerisation. Clinical detection of recurrence remains challenging, but DNA biomarkers in saliva may prove adjunctive to current diagnostic methods. However, primary site irradiation often leads to xerostomia. We assessed the ability of three saliva collection methods to generate DNA of sufficient quantity and quality for use in biomarker assays. Paired saliva samples were collected in OrageneTM vials and as saline mouthwash from non-irradiated (n=21) patients with oral squamous cell carcinoma (OSCC) and with OrageneTM sponge kits and as mouthwash from irradiated (n=24) patients with OSCC. Quantitative polymerase chain reaction demonstrated that OrageneTM vials contained DNA in significantly greater amounts (median: 122g) than mouthwash (median: 17g) (p=0.0001) in non-irradiated patients, while OrageneTM sponge kits (median: 4g) and mouthwash (median: 5.5g) generated comparable DNA concentrations from the irradiated cohort. All 90 samples contained DNA of sufficient quantity and quality for p16 promoter quantitative methylation specific PCR (qMSP). While OrageneTM vials contained the greatest amounts of DNA in this study, all three methods yielded sufficient DNA for the detection of DNA biomarkers using qMSP and the method of collection should be selected on the basis of patient compliance and oral competency.

Item Type:Article
Additional Information:Issue date: July 2013. Available online 12 October 2012.
Uncontrolled Keywords:oral cancer; biomarker; saliva; Collection method; Mouthwash; DNA; PCR
Subjects:R Medicine > RC Internal medicine > RC0254 Neoplasms. Tumors. Oncology (including Cancer)
R Medicine > RK Dentistry
Departments, Research Centres and Related Units:Academic Faculties, Institutes and Research Centres > Faculty of Medicine > School of Clinical Sciences
DOI:10.1016/j.bjoms.2012.09.017
Related URLs:
Refereed:Yes
Status:Published
ID Code:7695
Deposited On:21 Nov 2012 12:11
Last Modified:17 Jul 2013 14:45

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