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Molecular monitoring of the Leu-164 mutation of dihydrofolate reductase in a highly sulfadoxine/pyrimethamine-resistant area in Africa

Ochong, Edwin; Nzila, Alexis; Kimani, Serah; Kokwaro, Gilbert; Mutabingwa, Theonest; Watkins, William and Marsh, Kevin (2003) Molecular monitoring of the Leu-164 mutation of dihydrofolate reductase in a highly sulfadoxine/pyrimethamine-resistant area in Africa. Malaria Journal, 2 . Article Number: 46. ISSN 1475-2875

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Official URL: http://www.malariajournal.com/content/2/1/46

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Abstract

The selection of point mutation at codon 164 (from isoleucine to leucine) of the dihydrofolate reductase (DHFR) enzyme in Plasmodium falciparum is associated with high sulfadoxine / pyrimethamine (SP) resistance. Using the yeast expression system that allows the detection of dhfr allele present at low level, the presence of this mutation had previously been reported between 1998–1999 in Muheza, Tanzania, an area of high SP resistance. Eighty five P. falciparum isolates, obtained from the same area between 2002 and 2003, were analysed for the presence of Leu-164 mutation, using standard protocol based on PCR-RFLP. None of the isolates had the Leu-164 mutation.

Item Type:Article
Additional Information:Published: 15 December 2003. 3 pages (page numbers not for citation purposes).
Uncontrolled Keywords:PYRIMETHAMINE-SULFADOXINE RESISTANCE; PLASMODIUM-FALCIPARUM; CHLORPROGUANIL-DAPSONE; MALARIA; PARASITES; LAPDAP
Subjects:R Medicine > R Medicine (General)
Departments, Research Centres and Related Units:Academic Faculties, Institutes and Research Centres > Faculty of Medicine > School of Biomedical Sciences
DOI:10.1186/1475-2875-2-46
Publisher's Statement:© 2003 Ochong et al; licensee BioMed Central Ltd. This is an Open Access article: verbatim copying and redistribution of this article are permitted in all media for any purpose, provided this notice is preserved along with the article's original URL. This article is available from: http://www.malariajournal.com/content/2/1/46
Related URLs:
Refereed:Yes
Status:Published
ID Code:783
Deposited On:07 Jul 2008 10:23
Last Modified:19 May 2011 20:18

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