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Epithelial Na, K-ATPase expression is down-regulated in canine prostate cancer; a possible consequence of metabolic transformation in the process of prostate malignancy

Mobasheri, Ali; Fox, Richard; Evans, Iain; Cullingham, Fay; Martín-Vasallo, Pablo and Foster, Christopher S (2003) Epithelial Na, K-ATPase expression is down-regulated in canine prostate cancer; a possible consequence of metabolic transformation in the process of prostate malignancy. Cancer Cell International, 3 . Article Number: 8. ISSN 1475-2867

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Official URL: http://www.cancerci.com/content/3/1/8

Abstract

Background: An important physiological function of the normal prostate gland is the synthesis and secretion of a citrate rich prostatic fluid. In prostate cancer, citrate production levels are reduced as a result of altered cellular metabolism and bioenergetics. Na, K-ATPase is essential for citrate production since the inward Na+ gradients it generates are utilized for the Na+ dependent uptake of aspartate, a major substrate for citrate synthesis. The objective of this study was to compare the expression of previously identified Na, K-ATPase isoforms in normal canine prostate, benign prostatic hyperplasia (BPH) and prostatic adenocarcinoma (PCa) using immunohistochemistry in order to determine whether reduced citrate levels in PCa are also accompanied by changes in Na, K-ATPase expression. Results: Expression of Na, K-ATPase α1 and β1 isoforms was observed in the lateral and basolateral plasma membrane domains of prostatic epithelial cells in normal and BPH prostates. Canine kidney was used as positive control for expression of Na, K-ATPase α1 and γ isoforms. The α1 isoform was detected in abundance in prostatic epithelial cells but there was no evidence of α2, α3 or γ subunit expression. In advanced PCa, Na, K-ATPase α1 isoform expression was significantly lower compared to normal and BPH glands. The abundant basolateral immunostaining observed in normal and BPH tissue was significantly attenuated in PCa. Conclusion: The loss of epithelial structure and function and the transformation of normal epithelial cells to malignant cells in the canine prostate have important implications for cellular metabolism and are accompanied by a down regulation of Na, K-ATPase. , and 4

Item Type:Article
Additional Information:Published: 13 June 2003. 10 pages (page numbers not for citation purposes).
Uncontrolled Keywords:citrate; prostatic fluid; bioenergetics; altered cellular metabolism; aspartate; benign prostatic hyperplasia; prostatic adenocarcinoma; immunohistochemistry
Subjects:S Agriculture > SF Animal culture
Departments, Research Centres and Related Units:Academic Faculties, Institutes and Research Centres > Faculty of Medicine > School of Cancer Studies
Academic Faculties, Institutes and Research Centres > Faculty of Veterinary Science > Department of Veterinary Pathology
Academic Faculties, Institutes and Research Centres > Faculty of Veterinary Science > Department of Veterinary Preclinical Science
DOI:10.1186/1475-2867-3-8
Publisher's Statement:© 2003 Mobasheri et al; licensee BioMed Central Ltd. This is an Open Access article: verbatim copying and redistribution of this article are permitted in all media for any purpose, provided this notice is preserved along with the article's original URL. This article is available from: http://www.cancerci.com/content/3/1/8
Related URLs:
Refereed:Yes
Status:Published
ID Code:786
Deposited On:07 Jul 2008 11:21
Last Modified:30 Mar 2012 11:42

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