Skip navigation
   
 
Scholarly Communication
Contacts

Quantitative methylation analysis of resection margins and lymph nodes in oral squamous cell carcinoma

Shaw, Richard J.; Hall, Gillian L.; Woolgar, Julia A.; Lowe, Derek; Rogers, Simon N.; Field, John K.; Liloglou, Triantafillos and Risk, Janet M. (2007) Quantitative methylation analysis of resection margins and lymph nodes in oral squamous cell carcinoma. British Journal of Oral and Maxillofacial Surgery, 45 (8). pp. 617-622. ISSN 0266-4356

[img]
Preview
PDF
109Kb
[img]PDF (Four Figures)
Restricted to Repository staff only

150Kb
[img]Microsoft PowerPoint
1727Kb

Official URL: http://www.elsevier.com/wps/find/journaldescriptio...

Cited 23 times in WoS

Abstract

Resection is the preferred treatment for oral squamous cell carcinoma, and pathological staging of the resected specimen is crucial. The role of molecular biology in the diagnosis of minimal residual disease has not been fully investigated and may improve staging. Multiple adjacent specimens were taken from the tumour, the invasive front, the surgical margin, and the lymph nodes of 20 specimens from patients with oral cancer. Bisulphite-treated DNA from these specimens was assayed quantitatively with pyrosequencing methylation assays (PMA) of CpG islands within the gene promoters of the p16 and CYGB genes. Results were recorded with histopathological results, and compared with clinical outcome. Biological and technical replicates confirmed the reliability of the techniques. PMA upgraded 13 of the 20 surgical margins, 6 of which subsequently had a recurrent tumour. Not all of these recurrences were predicted and the effects of adjuvant treatment make firm conclusions difficult.

Item Type:Article
Additional Information:Available online 7 June 2007. Issue: December 2007.
Uncontrolled Keywords:Methylation; oral; squamous; cell; carcinoma; surgical; margin; pathology; pyrosequencing
Subjects:R Medicine > RK Dentistry
Departments, Research Centres and Related Units:Academic Faculties, Institutes and Research Centres > Faculty of Medicine > School of Dental Sciences
DOI:10.1016/j.bjoms.2007.04.015
Refereed:Yes
Status:Published
ID Code:94
Deposited On:07 Aug 2008 12:21
Last Modified:20 May 2011 19:41

Repository Staff Only: item control page

   
Search


Full text only
Peer reviewed only

Browse
Cross Archive Search
Find
Top 50 authors
Top 50 items
[more statistics]
 
   

These pages are maintained by Library Staff @ University of Liverpool Library

 

All pages © The University of Liverpool, 2004 | Disclaimer | Accessibility | Staff | Students