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Scholarly Communication

Quantitative methylation analysis of resection margins and lymph nodes in oral squamous cell carcinoma

Shaw, Richard J.; Hall, Gillian L.; Woolgar, Julia A.; Lowe, Derek; Rogers, Simon N.; Field, John K.; Liloglou, Triantafillos and Risk, Janet M. (2007) Quantitative methylation analysis of resection margins and lymph nodes in oral squamous cell carcinoma. British Journal of Oral and Maxillofacial Surgery, 45 (8). pp. 617-622. ISSN 0266-4356

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Resection is the preferred treatment for oral squamous cell carcinoma, and pathological staging of the resected specimen is crucial. The role of molecular biology in the diagnosis of minimal residual disease has not been fully investigated and may improve staging. Multiple adjacent specimens were taken from the tumour, the invasive front, the surgical margin, and the lymph nodes of 20 specimens from patients with oral cancer. Bisulphite-treated DNA from these specimens was assayed quantitatively with pyrosequencing methylation assays (PMA) of CpG islands within the gene promoters of the p16 and CYGB genes. Results were recorded with histopathological results, and compared with clinical outcome. Biological and technical replicates confirmed the reliability of the techniques. PMA upgraded 13 of the 20 surgical margins, 6 of which subsequently had a recurrent tumour. Not all of these recurrences were predicted and the effects of adjuvant treatment make firm conclusions difficult.

Item Type:Article
Additional Information:Available online 7 June 2007. Issue: December 2007.
Uncontrolled Keywords:Methylation; oral; squamous; cell; carcinoma; surgical; margin; pathology; pyrosequencing
Subjects:R Medicine > RK Dentistry
Departments, Research Centres and Related Units:Academic Faculties, Institutes and Research Centres > Faculty of Medicine > School of Dental Sciences
ID Code:94
Deposited On:07 Aug 2008 12:21
Last Modified:20 May 2011 19:41

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